Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages. In: Mass, E. (eds) Tissue-Resident Macrophages
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Published in Methods in Molecular Biology, 2023
In this protocol, we provide a detailed methodology on how to harvest alveolar macrophages and expand them in culture. We also provide a technical guidance on how to genetically modify this macrophage in culture by using lentiviral vector, which provides a potential application for studying gene function and gene and cellular therapy.
Recommended citation: Busch, C.JL., Subramanian, S., Linares, J., Favret, J., Yuda, R.A.A., Sieweke, M.H. "Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages. In: Mass, E. (eds) Tissue-Resident Macrophages." Methods in Molecular Biology, vol 2713. 231-251(2024).
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