Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

Authors : Clara Jana-Lui Busch, Sethuraman Subramanian, Javier Linares, Jérémy Favret, Ridzky Anis Advent Yuda, Michael H. Sieweke — excerpt: “Alveolar Macrophage Culture and Genetic Modification Protocol
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Abstract Alveolar macrophages (AM) are resident macrophages of the lung and play important roles in the maintenance of tissue homeostasis as well as host defense. Here, we describe how they can be harvested from murine lungs, expanded in vitro, and transduced with lentiviral vectors. — external_url: https://link.springer.com/protocol/10.1007/978-1-0716-3437-0_16 —